LOCUS       sequence-54186-e        1458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Tait SW, Parsons MJ, Llambi F, Bouchier-Hayes L, Connell S, 
            Munoz-Pinedo C, Green DR
  TITLE     Resistance to caspase-independent cell death requires persistence of
            intact mitochondria.
  JOURNAL   Dev Cell. 2010 May 18;18(5):802-13.
  PUBMED    20493813
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2010-05-18"; volume: "18(5)"; pages: "802-13"
COMMENT     Sequence Label: pSmac-GFP
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      733..742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(739..741,742..744,745..1458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(784..805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1045..1064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1392..1413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcggctc tgaagagttg gctgtcgcgc agcgtaactt cattcttcag gtacagacag
       61 tgtttgtgtg ttcctgttgt ggctaacttt aagaagcggt gtttctcaga attgataaga
      121 ccatggcaca aaactgtgac gattggcttt ggagtaaccc tgtgtgcggt tcctattgca
      181 cagaaatcag agcctcattc ccttagtagt gaagcattga tgaggagagc agtgtctttg
      241 gtaacagata gcacctctac ctttctctct cagaccacat atgcgttgat tgaagctatt
      301 actgaatata ctaaggctgt ttatacctta acttctcttt accgacaata tacaagttta
      361 cttgggaaaa tgaattcaga ggaggaagat gaagtgtggc aggtgatcat aggagccaga
      421 gctgagatga cttcaaaaca ccaagagtac ttgaagctgg aaaccacttg gatgactgca
      481 gttggtcttt cagagatggc agcagaagct gcatatcaaa ctggcgcaga tcaggcctct
      541 ataaccgcca ggaatcacat tcagctggtg aaactgcagg tggaagaggt gcaccagctc
      601 tcccggaaag cagaaaccaa gctggcagaa gcacagatag aagagctccg tcagaaaaca
      661 caggaggaag gggaggagcg ggctgagtcg gagcaggagg cctacctgcg tgaggatgcg
      721 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      781 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc
      841 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      901 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      961 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1021 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1081 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1141 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1201 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac
     1261 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1321 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1381 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1441 gacgagctgt acaagtaa
//
