LOCUS       sequence-54196-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII324
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    47..154
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     63..79
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     64..80
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(162..181)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(163..181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(188..205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(188..204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(197..219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      345..773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(432..451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     642..663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc
       61 cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac
      121 tagttctaga gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt
      181 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      241 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      301 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
      361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      781 aatat
//
