LOCUS       sequence-54197-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII425
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    31..138
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(105..121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(146..165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(147..165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(175..192)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(175..191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(184..206)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      332..712
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(419..438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     629..650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc
      121 gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
      181 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      241 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      301 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
      361 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      421 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      481 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      541 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      601 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      661 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      721 ttaaaa
//
