LOCUS       sequence-54198-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3-AKAR2-CR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..766)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat tcttacttgt
       61 acagctcgtc catcccacca ccaagcccgg cgaacttggc aactgcgtgt tcgcgttgta
      121 ctacgaacat ttcattgtca ctttcctcta acctttccag gcggtgatca acggcatgga
      181 taccgggcat cttgatgttc ccgacggtct tttttgacct gtaagttgtt acgaaagagc
      241 aagacagatg gccaccacca tcaactttca gtgccatatg agtgtatccc ctcagaccac
      301 catctgctgg atacatcatc tctgtattag gctcccaacc cttggtcttc ttctgcatca
      361 cgggaccatt ggagggaaag tttacccctc tgacttggac gtggtaaacg agacagccat
      421 cctcaaggct ggtgtcctgc atgacggtga cgactccacc atcttcgtat ctcgtaactc
      481 tttcccaagt aaaaccctca ggaaaggact gtttaaagaa atcaggaatg cctttcgggt
      541 acttgataaa agtacggctg ccatacatga acgacgtggc aagaatgtca aaggcaaatg
      601 gcaggggtcc tccctcgatg actttgatcc tcatggtttg agttcccatg tacggattgc
      661 cttctccttc acctgtgcat ttgaattggt ggccgttgac cgaaccttcc atgaccacct
      721 tcatacgcat attttccttg atcagctctt cgcccttaga caccatgagc tcgctgccgc
      781 cggtgccgcc gtccaccagg gtggcgcgac gcaggccctt ggtgctgccc tcgccgctgc
      841 ctggcttacc tgcagagcga tcaactttgt tct
//
