LOCUS       sequence-54209-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pCAGGS-Raichu-RhoA-CR
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        241..345
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(564..583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatactccc ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
       61 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      121 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
      181 actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg
      241 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      301 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      361 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      421 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      481 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      541 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      601 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      661 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      721 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      781 ct
//
