LOCUS       sequence-54258-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  TITLE     pFA6-yEGFP1-HIS3MX6
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS413-GAL1-yEGFP1
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..740)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGIDELYK"
ORIGIN
        1 attcgcttat ttagaagtgg cgcgccttat ttgtacaatt catcaatacc atgggtaata
       61 ccagcagcag taacaaattc taacaagacc atgtggtctc tcttttcgtt tggatctttg
      121 gataaggcag attgagtgga taagtaatgg ttgtctggta acaagactgg accatcacca
      181 attggagtat tttgttgata atggtcagct aattgaacag aaccatcttc aatgttgtgt
      241 ctaattttga agttaacttt gataccattc ttttgtttgt cagccatgat gtaaacattg
      301 tgagagttat agttgtattc caatttgtga cctaaaatgt taccatcttc tttaaaatca
      361 atacctttta attcgattct attaactaag gtatcacctt caaacttgac ttcagctctg
      421 gtcttgtagt taccgtcatc tttgaaaaaa atagttcttt cttgaacata accttctggc
      481 atggcagact tgaaaaagtc atgttgtttc atatgatctg ggtatctaga aaaacattga
      541 acaccataag ttaaagtagt gactaaggtt ggccatggaa ctggcaattt accagtagta
      601 caaataaatt ttaaggtcaa tttaccgtaa gtagcatcac cttcaccttc accggagaca
      661 gaaaatttgt gaccattaac atcaccatct aattcaacca aaattgggac aacaccagtg
      721 aataattctt cacctttaga c
//
