LOCUS       sequence-54271-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pCA-G-intron-G
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(63..84)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(309..342)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(494..513)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccgatatc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggttggtatc
      301 aaagatctat aacttcgtat agcatacatt atacgaagtt atggttacaa gacaggttta
      361 aggagaccaa tagaaactgg gcatgtggag acagagaaga ctcttgggtt tctgataggc
      421 actgactctc tctgcctatt ggtctatttt cccaccctta gggtacgtcc aggagcgcac
      481 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      541 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      601 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      661 gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg gcggcgtgca
      721 gctcgccgac cactaccagc agaacacccc catcggcgac ggcccc
//
