LOCUS       sequence-54284-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2210
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catctcgcaa taaataagta ttttactggt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc
      121 ttacgtaaga ccattatcaa cgaaagcctg aacttcaaaa ttcgcgacca gctggtggtc
      181 ggacagctga tccccgactg ctacgtggaa cttgagaaga tcatcctgag cgagcgcaag
      241 aatgtgccta tcgagtttcc agtgatcgat cgcaaacgtc tcctccagct cgtgcgtgag
      301 aatcagctgc agctcgacga gaacgagctg cctcatgctg tgcacttcct taacgagtca
      361 ggtgtcctcc tgcacttcca ggacccggct ctccagctga gtgacctcta tttcgtggag
      421 ccaaagtggc tctgtaaaat tatggctcaa atcctcactg tcaaggtaga gggctgtcca
      481 aagcacccaa agggcatcat ctcgcgtcgc gacgtggaaa agtttctctc taagaagcgc
      541 aagtttccca agaactacat gagccagtac tttaaactgt tggagaagtt ccagatcgct
      601 ctgcctatcg gcgaagagta cctgttggtt ccttcctccc tgtccgatca ccgccccgtg
      661 attgagctgc ctcactgcga gaacagcgag attatcattc gcctatacga gatgccgtac
      721 tttccgatgg gcttctggtc aaggctcatc aatcgtctgc ttgaaatctc cccatatatg
      781 ctgagcggtc gtgaacgcgc tctgaggcca aaccggatgt actggcgcca ggggatttac
      841 ttgaactggt cccctgaggc ttattgtctg
//
