LOCUS       sequence-54295-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB12 LRRK2_BC_1847_2210_K1906M
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             415..432
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagca ggaggaattc
       61 atgaagcatc accaccatca ccatctcgag tccgataaga taatccacct gactgacgac
      121 tccttcgata ccgacgtgct gaaggcagac ggtgctatcc tcgttgactt ctgggcccac
      181 tggtgtggtc cttgcaagat gatagctcct attcttgacg agatcgcaga cgaataccaa
      241 ggtaagctga ccgttgctaa gctgaacatt gatcataacc ctggcaccgc acctaaatac
      301 ggcatccgtg gcatcccaac tttgctcctg ttcaagaatg gtgaagtagc agctaccaag
      361 gtaggcgcac tgtctaaagg ccagctgaaa gaatttctgg acgccaactt agctttagtt
      421 cctcgcggat ccaggctgac catccccatc tcccagattg cacccgacct tatcctcgct
      481 gacctgcctc gcaacatcat gctgaacaat gacgagctgg agtttgaaca agcacccgag
      541 ttcctgctag gcgacggctc tttcggttca gtttaccgtg ctgcttacga gggtgaagag
      601 gttgccgtga tgatttttaa caagcacact tcgctccgtc tgctgcgtca ggagcttgtg
      661 gttctctgcc acctgcacca cccttccctg atctccctgc tggccgccgg cattaggcct
      721 cgcatgctag tgatggaact ggccagtaag ggttcattgg accgcctgct gcagcaggac
      781 aaggctagcc tgaccccgta cgctccagca ccgtatcgcc ctgcatgtgg ccgac
//
