LOCUS       sequence-54316-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pLN
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(219..238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(220..238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(245..262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(245..261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(254..276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             399..701
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     580..599
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
       61 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aataacttcg
      121 tataatgtat gctatacgaa gttatagatc ttcctgaatt ctgcagatat ccatcacact
      181 ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag tcgtattaca
      241 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      361 atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt tacacctata
      421 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgccgg
      481 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
      541 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
      601 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
      661 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc atgagattat
      721 caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct
      781 gacc
//
