LOCUS       sequence-54317-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pFLN
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    116..149
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(156..189)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(265..284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(266..284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(291..308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(291..307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(300..322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             445..747
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     626..645
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaataac
      121 ttcgtataat gtatgctata cgaagttata gatccgaagt tcctatactt tctagagaat
      181 aggaacttcg gagatcttcc tgaattctgc agatatccat cacactggcg gccgctcgag
      241 catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      421 cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt
      481 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg
      541 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
      601 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      661 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      721 attaacctga tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc
      781 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
      841 gtcagctact gggctatctg gacaaggga
//
