LOCUS       sequence-54326-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Austin BP, Tozser J, Bagossi P, Tropea JE, Waugh DS
  TITLE     The substrate specificity of Metarhizium anisopliae and Bos taurus 
            carboxypeptidases A: insights into their use as tools for the 
            removal of affinity tags.
  JOURNAL   Protein Expr Purif. 2011 May;77(1):53-61. Epub 2010 Nov 10.
  PUBMED    21073956
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2011-05"; volume: "77(1)"; pages: "53-61. Epub 
            2010 Nov 10"
COMMENT     Sequence Label: p1177-X1-8
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..154
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(162..179)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag tacttctcga
      121 caagctatca ccactttgta caagaaagtt ggctagcgac gatggtgatg gtgatggtga
      181 ctcatctgct ggaagagatg catggcacca gcaaagctct cctctccaga cggtatgatc
      241 tggtccggtg gcaggacaaa cccataccta cctttgtcac gcagttcgat tgtgaagaca
      301 ttgtcggctt taagaacgtc ttggacccaa tcaatagagc caccggcaac ttggtagatg
      361 acattgcaaa ctggaccata tgcgaaggtg gttccgtgga cactcctcat agcgtcagaa
      421 gcaccctttg ccagagcttg caaggcagca ttgttgggcg tccttgcgct gcacgagtac
      481 ccgtatggtg tcatgaacag ctgagaatac gagtgccagt cgatgtatag cttgacgcct
      541 tggctcttct tgatcttgtc gaggaaggcg atgtacgact tgacttctgg gctgttgccc
      601 gcagatgcac cccgatacgt ctcagtacaa gggttcgttg atgacccagg gccatcccac
      661 ttgtatggcc agttgcgatt gggatccgtg ccgagacagc tcgagccctg gttgcgagac
      721 cggttcttcc tccacatgcg gtctgacgac tgcgtgtact tgaagccgtc aacattgaca
      781 atggggaaca tgtagaagtc gtacttg
//
