LOCUS       sequence-54334-e       10844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 10844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pDM291-tetR-tup11-Delta-70
FEATURES             Location/Qualifiers
     source          1..10844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1742..2536)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     primer_bind     complement(4269..4288)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4270..4288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4399..4778
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4779..4982
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4933..4953
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4979..5003
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5084..5704
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5157..5176)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             7154..7165
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(7487..7504)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7487..7503)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7496..7518)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      7666..7853
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7721..7745)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8587..8604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8758..9346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8838..8857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9517..10308,10309..10377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10140..10159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10378..10482)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10550..10568
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10606..10628)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10728..10747
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10828..10844,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(10828..10844,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgatccg ctgttccagt caatcagggt attgaagctc atggtcttta ctccatcaca
       61 gggttccgcc ttatccggcc tacagaaccc aaaatatcaa cgcattacgt aggcctgata
      121 agcgcagcgc catcaggcgt cagatcactc catcatcttc tcgatcggca gtaccagaat
      181 cgagctggcg ccaagcgctt tcagtttctc catggtttcc cagaacaacg tttcgctgct
      241 gaccatgtgc atcgccacgc gctgttgctc gcctgccagc ggcagaattg tcggcctttc
      301 ggcgcctggc agcagggcga taacctcttc caggcgttca cttggcgcgt gcatcatgat
      361 gtatttcgat tcgcgcgcct gaatcacgcc ctgaatacgg gtcagcaatt tatcgatcag
      421 ctcttgcttg ctctgtgcca tctcaccgtc gcgctgaatc agacaggctt tagagcggta
      481 gataacttcg acttcacgca ggccgttagc ttcaagcgtc gcgccggtag agaccaaatc
      541 gcagatagcg tcggccagcc ccgcgcgcgg cgcgacttcg acagaaccat ttaacagaca
      601 cgatttaaaa gagacgcctt tctggtcgag gtagcgtttg aggaggtgcg gatatgaggt
      661 agcgatacgt ttaccgtcca gcgcggccgg gccgtcccag gcttcgtcaa ccggtgttgc
      721 cagcgataaa cggcagccgc cgaagtcaag acggcgcagg gttaaatagc gtggatcttc
      781 gccctgtgcg cggcggttga gtagctcttc ttccagcacg ttttcgccga taataccgag
      841 atcgaccacg ccatccatta ccagacccgg aatgtcatca tcacgcacgc gcaggatatc
      901 aatcggcatg ttttccgcca tcgcaatcag gcgctgagtg tgtaaattaa tttttatgcc
      961 gcagcgggcc agcaattctc gtgaatcatc gcttaaacgg cctgatttct gaatagctat
     1021 gcgtaagcgg gtgttgtcta acattctgcg ttcctcttta tcctgtctga accggtctgt
     1081 atcgcgcgcc aaaaaaaaag cccccggaag atgatcttcc gggggctttc tcatgcgttc
     1141 atgcaccact ggaagatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
     1201 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
     1261 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcaagct tgtgatattg
     1321 acgaaacttt ttgacatcta atttattctg ttccaacacc aatgtttata accaagtttt
     1381 atcttgtttg tctacatggt attttacatt catctacata catctttcat tggctttgta
     1441 catagttatc attacaagtc taaaaaaatt cactcttttc ttattcaatg tcaatccaag
     1501 agaaaagatt gtggtaatgt tgtaggagca tgtttaataa attactatag caaattactt
     1561 tttattccca aggtgtttat ctataatagt taatatttta gtcgctacat aaaattttac
     1621 caaagagtac ttgtatacta attctaaatg ccttctgaca taaaacgcct aggaaaacaa
     1681 acgcaaacaa ggcatcgact ttttcaataa ccaaccaaaa aaattttaca ttagtctttt
     1741 tttaatgctg agaaagtctt tgctgatatg ccttccaacc agcttctcta tatctcttgg
     1801 cttcgacaac aggattacga ccagctccat agactccacg accaacaatg atgatatcgc
     1861 taccgcagtt tacaatcact tcttcaggag tacgatattg ctgtcccagc ccgtctcctt
     1921 taacatccaa gccgatacca ggggacatag ttatgtagtc gctttgaagg ttaggaaatc
     1981 gacgaccagc tataaagcca aagcaaaaat cggtatgctt ctcaaaccat tctaaggttt
     2041 tctctgtgta ggaaccagta gccaaagagc ctttggaaga catttcagcc aaaagcaaga
     2101 gaccacgtcc caaaggtaaa ccaacttctt tgaggccttg tataataccc tcgcctggca
     2161 ctgtatggca atttgtgata tgagcccaag aagcaatttt gtacacacca gatgcatatt
     2221 gtagcttgac ggtatttcca atgtctgcga atttgcgatc ctcaaagata agaaaacgat
     2281 gctttttacc taaggccacc agtttttcta ccatatcctg gtcgaaatcc tcgacaacgt
     2341 caatatgtgt cttgataaca cagacatagg gtccaatttt atctaccaat tctaagattt
     2401 cggatttctt cgtcaaatcg accgcgactg acaagttgct ttgcttttct tccatcaaag
     2461 ccaacaattc cttggcaatg ggatttttca tcccctcagc tctagctgaa tagctttgaa
     2521 atactctagc atccataact ttgcttttaa acctttaatt tcgatccaag caaaaaagag
     2581 gttcttggta ggacaatacg gtaagaaaac acgacatgtg cagagatgcc gacgaagcat
     2641 agttaaactg ggatggtaaa atcaattaag aatttataaa gacaaaattg tataagtctc
     2701 taaaacatct taattatacc tcacagaact atctaaaata tattcacaaa gtgcaaacat
     2761 tatcatgaaa aagaaccatt ttaatttaaa gcaagggcat taaggcttat ttacagaatt
     2821 tcttactttt gtaaagatta taaggctgat tatctttttc accatgccaa aaattacaca
     2881 agatagaatg gatgtttgaa attaaacgtg agtatacaaa caaatacact aggtaaatcg
     2941 aaacattttt ttctccatta agtaacaaat tcctatttag agaaagaatg ctgagtagat
     3001 taaataatct atacaaactt ttttaacaca aatgcataca tatagccagt gggatttgta
     3061 gctaagctta tcgatgataa gctgtcaaac atgagaatta attcagatcc gctgttccag
     3121 tcaatcaggg tattgaagct catggtcttt actccatcac agggttccgc cttatccggc
     3181 ctacagaacc caaaatatca acgcattacg taggcctgat aagcgcagcg ccatcaggcg
     3241 tcagatcact ccatcatctt ctcgatcggc agtaccagaa tcgagctggc gccaagcgct
     3301 ttcagtttct ccatggtttc ccagaacaac gtttcgctgc tgaccatgtg catcgccacg
     3361 cgctgttgct cgcctgccag cggcagaatt gtcggccttt cggcgcctgg cagcagggcg
     3421 ataacctctt ccaggcgttc acttggcgcg tgcatcatga tgtatttcga ttcgcgcgcc
     3481 tgaatcacgc cctgaatacg ggtcagcaat ttatcgatca gctcttgctt gctctgtgcc
     3541 atctcaccgt cgcgctgaat cagacaggct ttagagcggt agataacttc gacttcacgc
     3601 aggccgttag cttcaagcgt cgcgccggta gagaccaaat cgcagatagc gtcggccagc
     3661 cccgcgcgcg gcgcgacttc gacagaacca tttaacagac acgatttaaa agagacgcct
     3721 ttctggtcga ggtagcgttt gaggaggtgc ggatatgagg tagcgatacg tttaccgtcc
     3781 agcgcggccg ggccgtccca ggcttcgtca accggtgttg ccagcgataa acggcagccg
     3841 ccgaagtcaa gacggcgcag ggttaaatag cgtggatctt cgccctgtgc gcggcggttg
     3901 agtagctctt cttccagcac gttttcgccg ataataccga gatcgaccac gccatccatt
     3961 accagacccg gaatgtcatc atcacgcacg cgcaggatat caatcggcat gttttccgcc
     4021 atcgcaatca ggcgctgagt gtgtaaatta atttttatgc cgcagcgggc cagcaattct
     4081 cgtgaatcat cgcttaaacg gcctgatttc tgaatagcta tgcgtaagcg ggtgttgtct
     4141 aacattctgc gttcctcttt atcctgtctg aaccggtctg tatcgcgcgc caaaaaaaaa
     4201 gcccccggaa gatgatcttc cgggggcttt ctcatgcgtt catgcaccac tggaagatct
     4261 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttgggg gtcgaggagc
     4321 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
     4381 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     4441 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     4501 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     4561 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cgctaaactg
     4621 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     4681 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     4741 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     4801 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4861 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4921 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4981 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     5041 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt catatgtcta gattagataa
     5101 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     5161 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     5221 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     5281 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     5341 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     5401 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     5461 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     5521 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     5581 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     5641 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     5701 atctgttagt atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt
     5761 tcaggcaggg atgttctcac ctaagcttag aacctttacc aaaggtgatg cggagagatg
     5821 ggtaagcggt acctacgaaa aagatatcaa caagttgaag cgagagcttg aagaattagg
     5881 tgtcgaagcg aataccgcaa gttatagaaa tcgtggagaa agatctgaac ttgccgcttc
     5941 taataatcaa gttacccata ttgatcagga acatccctct caaaccaaat ctacttctca
     6001 gcctccaagt aaccatctac ctgcctttca gcaaatccct ccaattcacc aatcagctta
     6061 tcctcaaaat aatgttgctg aagtattgat gcctccaatt cctccttcag tagaggcttc
     6121 tagcgggcaa aattttaatc agggaattgc ttctcaaaat cccgcgatat ctacatccaa
     6181 tttacctagt actacacctt tatatatccc tcctgttaat tacggagcaa atcaggtttc
     6241 tcaacaacca aatcctcaat tgcctggtgt ttccaattat tataatccaa gtgctacttc
     6301 aaaacctgct gtgaatgtcc agccaccgcg tattcctacc aaagcaactc cttcggctga
     6361 accttctatg actgcttctg cgaatgctgg ttctatatcc caagcgggtc ctgatggaga
     6421 atatcaggga agagaacaga ttgctccagt ttcagataca gaagctgcta gaaagacaac
     6481 ttctcagagt tggtacgtca cttataaccc agcatgcaaa agggttttta atattaatct
     6541 cgtgcacaca ctagagcatc ccagtgttgt ttgctgcgtt aagtttagta acaatggcaa
     6601 gtatttagca actggctgta accaggccgc taatgtcttt gatgttcaga ctggcaaaaa
     6661 actttttact ttgcatgaag aatcacctga tccttcccgt gatttgtatg tgagaaccat
     6721 tgctttcagc cctgatggta agtatttagt cacgggaaca gaagatcgtc aaataaaact
     6781 ctgggatctc tcaactcaaa aagttcgtta cgtattttca ggtcatgaac aagatatcta
     6841 ttccttagat tttagccata atgggcgatt catcgtctct ggtagtggcg atcgaactgc
     6901 acgactctgg gatgtggaga caggtcaatg tattttaaag ttagaaattg agaatggtgt
     6961 gacggcaata gccatctctc caaatgatca attcattgca gttggtagtc tagatcagat
     7021 aattcgagta tggtctgtgt cgggtacttt ggttgaacga ttggaaggtc ataaggagag
     7081 tgtttattct attgcctttt cgcccgacag cagcattctt cttagtggaa gtcttgataa
     7141 gactatcaaa gtctgggaat tacaagcaac tcgttcagtt ggcctttcag ctattaagcc
     7201 tgaaggtatt tgcaaagcca cctatacagg acatactgat tttgttcttt cagtagctgt
     7261 ttcacccgac agccgttggg gattaagcgg atcgaaggat agaagtatgc aattctggga
     7321 tttgcaaaca ggccaatctt atctcacttg tcaaggacac aagaattcag ttatttccgt
     7381 atgctttagc ccagatggta ggcaatttgc ttcgggaagt ggtgatttac gtgctcgcat
     7441 atggtcaatt gaccctgcat ctccttgagg taccgagctc gaattcactg gccgtcgttt
     7501 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7561 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7621 tgcgcagcct gaatggcgaa tggcgcccgc gccacttcta aataagcgaa tttcttatga
     7681 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
     7741 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
     7801 ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc
     7861 gctagggggt gcaaataggt tttaaaattg ctccaatcac atgttcttaa gaaaaaaacg
     7921 tcaaaagaaa tctaagtatt cccaaaataa ttcctcctga catgagaaga ataaaaaaca
     7981 ctaagatgca ttgtcagtat gaaacacaag ccaaagttgc taacaatagg atcaattctt
     8041 acttactggc aatgttacga ggaatcttgg taggaggatg tttggcaaca taacgttctt
     8101 ttgaatttgt ttttacacga cgagtaatgt tcttctgaaa ctgtgtgttt gcacgacgct
     8161 gagagggagt ttgaaccgtc taaaagacat agttaggatc catgatgcac agatttctaa
     8221 agataaaact acttacaggc atgttggttt attaaaaaag aaaaacagga atgataagag
     8281 ggaaagaata cgtgtacaga gcaagtgtgg aaagatatgc tgaagacgtt tgtatgaaca
     8341 atgccaagct gtaaagctag actttcaaag tacacactaa tacgttatac tatataccaa
     8401 acaaaagggt acgaacttac agtgtttacc aatgtcagca atactacact acgctatgat
     8461 acactacgtt gtgtatcact atatgtcaca tgttctaatt atatatcgta ccatgcccaa
     8521 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9181 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10501 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10561 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10621 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10681 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    10741 gattgtactg agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata
    10801 cataacctta tgtatcatac acatacgatt taggtgacac tata
//
