LOCUS       sequence-54336-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72110-BBa_J72094
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(185..228)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
       61 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      121 tgaattgcca ttatcaacaa gtttgtacaa aaaagcaggc tccgaattgg tatcacgagg
      181 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcatga
      241 gatctctatg ctactccatc gagccgtcaa ttgtctgatt cgttaccaat tatgacaact
      301 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
      361 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
      421 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
      481 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga cgtgacagac
      541 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
      601 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
      661 actcgttaat cgcttccatg cgccgcagca acaattgctc aagcagattt atcgccagca
      721 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
      781 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
      841 agttaagcca ttcatgccag taggcgcgcg gacgaaa
//
