LOCUS       sequence-54338-e       10842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Zilio N, Wehrkamp-Richter S, Boddy MN
  TITLE     A new versatile system for rapid control of gene expression in the 
            fission yeast Schizosaccharomyces pombe.
  JOURNAL   Yeast. 2012 Aug 29. doi: 10.1002/yea.2920.
  PUBMED    22968950
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2012
            Aug 29. doi: 10.1002/yea.2920."
COMMENT     Sequence Label: pDM291-tetR'-tup11-Delta-70
FEATURES             Location/Qualifiers
     source          1..10842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1742..2536)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     primer_bind     complement(4269..4288)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4270..4288)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4399..4778
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4779..4982
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4933..4953
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4979..5003
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5082..5702
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLKGESWQDFLRNNAKSFRCALLSHRNGAKVHSDTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5155..5174)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             7152..7163
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(7485..7502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7485..7501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7494..7516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      7664..7851
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7719..7743)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8585..8602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8756..9344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8836..8855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9515..10306,10307..10375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10138..10157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10376..10480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10548..10566
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10604..10626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10726..10745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10826..10842,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(10826..10842,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgatccg ctgttccagt caatcagggt attgaagctc atggtcttta ctccatcaca
       61 gggttccgcc ttatccggcc tacagaaccc aaaatatcaa cgcattacgt aggcctgata
      121 agcgcagcgc catcaggcgt cagatcactc catcatcttc tcgatcggca gtaccagaat
      181 cgagctggcg ccaagcgctt tcagtttctc catggtttcc cagaacaacg tttcgctgct
      241 gaccatgtgc atcgccacgc gctgttgctc gcctgccagc ggcagaattg tcggcctttc
      301 ggcgcctggc agcagggcga taacctcttc caggcgttca cttggcgcgt gcatcatgat
      361 gtatttcgat tcgcgcgcct gaatcacgcc ctgaatacgg gtcagcaatt tatcgatcag
      421 ctcttgcttg ctctgtgcca tctcaccgtc gcgctgaatc agacaggctt tagagcggta
      481 gataacttcg acttcacgca ggccgttagc ttcaagcgtc gcgccggtag agaccaaatc
      541 gcagatagcg tcggccagcc ccgcgcgcgg cgcgacttcg acagaaccat ttaacagaca
      601 cgatttaaaa gagacgcctt tctggtcgag gtagcgtttg aggaggtgcg gatatgaggt
      661 agcgatacgt ttaccgtcca gcgcggccgg gccgtcccag gcttcgtcaa ccggtgttgc
      721 cagcgataaa cggcagccgc cgaagtcaag acggcgcagg gttaaatagc gtggatcttc
      781 gccctgtgcg cggcggttga gtagctcttc ttccagcacg ttttcgccga taataccgag
      841 atcgaccacg ccatccatta ccagacccgg aatgtcatca tcacgcacgc gcaggatatc
      901 aatcggcatg ttttccgcca tcgcaatcag gcgctgagtg tgtaaattaa tttttatgcc
      961 gcagcgggcc agcaattctc gtgaatcatc gcttaaacgg cctgatttct gaatagctat
     1021 gcgtaagcgg gtgttgtcta acattctgcg ttcctcttta tcctgtctga accggtctgt
     1081 atcgcgcgcc aaaaaaaaag cccccggaag atgatcttcc gggggctttc tcatgcgttc
     1141 atgcaccact ggaagatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
     1201 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
     1261 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcaagct tgtgatattg
     1321 acgaaacttt ttgacatcta atttattctg ttccaacacc aatgtttata accaagtttt
     1381 atcttgtttg tctacatggt attttacatt catctacata catctttcat tggctttgta
     1441 catagttatc attacaagtc taaaaaaatt cactcttttc ttattcaatg tcaatccaag
     1501 agaaaagatt gtggtaatgt tgtaggagca tgtttaataa attactatag caaattactt
     1561 tttattccca aggtgtttat ctataatagt taatatttta gtcgctacat aaaattttac
     1621 caaagagtac ttgtatacta attctaaatg ccttctgaca taaaacgcct aggaaaacaa
     1681 acgcaaacaa ggcatcgact ttttcaataa ccaaccaaaa aaattttaca ttagtctttt
     1741 tttaatgctg agaaagtctt tgctgatatg ccttccaacc agcttctcta tatctcttgg
     1801 cttcgacaac aggattacga ccagctccat agactccacg accaacaatg atgatatcgc
     1861 taccgcagtt tacaatcact tcttcaggag tacgatattg ctgtcccagc ccgtctcctt
     1921 taacatccaa gccgatacca ggggacatag ttatgtagtc gctttgaagg ttaggaaatc
     1981 gacgaccagc tataaagcca aagcaaaaat cggtatgctt ctcaaaccat tctaaggttt
     2041 tctctgtgta ggaaccagta gccaaagagc ctttggaaga catttcagcc aaaagcaaga
     2101 gaccacgtcc caaaggtaaa ccaacttctt tgaggccttg tataataccc tcgcctggca
     2161 ctgtatggca atttgtgata tgagcccaag aagcaatttt gtacacacca gatgcatatt
     2221 gtagcttgac ggtatttcca atgtctgcga atttgcgatc ctcaaagata agaaaacgat
     2281 gctttttacc taaggccacc agtttttcta ccatatcctg gtcgaaatcc tcgacaacgt
     2341 caatatgtgt cttgataaca cagacatagg gtccaatttt atctaccaat tctaagattt
     2401 cggatttctt cgtcaaatcg accgcgactg acaagttgct ttgcttttct tccatcaaag
     2461 ccaacaattc cttggcaatg ggatttttca tcccctcagc tctagctgaa tagctttgaa
     2521 atactctagc atccataact ttgcttttaa acctttaatt tcgatccaag caaaaaagag
     2581 gttcttggta ggacaatacg gtaagaaaac acgacatgtg cagagatgcc gacgaagcat
     2641 agttaaactg ggatggtaaa atcaattaag aatttataaa gacaaaattg tataagtctc
     2701 taaaacatct taattatacc tcacagaact atctaaaata tattcacaaa gtgcaaacat
     2761 tatcatgaaa aagaaccatt ttaatttaaa gcaagggcat taaggcttat ttacagaatt
     2821 tcttactttt gtaaagatta taaggctgat tatctttttc accatgccaa aaattacaca
     2881 agatagaatg gatgtttgaa attaaacgtg agtatacaaa caaatacact aggtaaatcg
     2941 aaacattttt ttctccatta agtaacaaat tcctatttag agaaagaatg ctgagtagat
     3001 taaataatct atacaaactt ttttaacaca aatgcataca tatagccagt gggatttgta
     3061 gctaagctta tcgatgataa gctgtcaaac atgagaatta attcagatcc gctgttccag
     3121 tcaatcaggg tattgaagct catggtcttt actccatcac agggttccgc cttatccggc
     3181 ctacagaacc caaaatatca acgcattacg taggcctgat aagcgcagcg ccatcaggcg
     3241 tcagatcact ccatcatctt ctcgatcggc agtaccagaa tcgagctggc gccaagcgct
     3301 ttcagtttct ccatggtttc ccagaacaac gtttcgctgc tgaccatgtg catcgccacg
     3361 cgctgttgct cgcctgccag cggcagaatt gtcggccttt cggcgcctgg cagcagggcg
     3421 ataacctctt ccaggcgttc acttggcgcg tgcatcatga tgtatttcga ttcgcgcgcc
     3481 tgaatcacgc cctgaatacg ggtcagcaat ttatcgatca gctcttgctt gctctgtgcc
     3541 atctcaccgt cgcgctgaat cagacaggct ttagagcggt agataacttc gacttcacgc
     3601 aggccgttag cttcaagcgt cgcgccggta gagaccaaat cgcagatagc gtcggccagc
     3661 cccgcgcgcg gcgcgacttc gacagaacca tttaacagac acgatttaaa agagacgcct
     3721 ttctggtcga ggtagcgttt gaggaggtgc ggatatgagg tagcgatacg tttaccgtcc
     3781 agcgcggccg ggccgtccca ggcttcgtca accggtgttg ccagcgataa acggcagccg
     3841 ccgaagtcaa gacggcgcag ggttaaatag cgtggatctt cgccctgtgc gcggcggttg
     3901 agtagctctt cttccagcac gttttcgccg ataataccga gatcgaccac gccatccatt
     3961 accagacccg gaatgtcatc atcacgcacg cgcaggatat caatcggcat gttttccgcc
     4021 atcgcaatca ggcgctgagt gtgtaaatta atttttatgc cgcagcgggc cagcaattct
     4081 cgtgaatcat cgcttaaacg gcctgatttc tgaatagcta tgcgtaagcg ggtgttgtct
     4141 aacattctgc gttcctcttt atcctgtctg aaccggtctg tatcgcgcgc caaaaaaaaa
     4201 gcccccggaa gatgatcttc cgggggcttt ctcatgcgtt catgcaccac tggaagatct
     4261 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttgggg gtcgaggagc
     4321 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
     4381 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     4441 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     4501 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     4561 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cgctaaactg
     4621 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     4681 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     4741 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     4801 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4861 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4921 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4981 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     5041 gaagacaccg ggaccgatcc agcctccgcg gccgaattca tatgtctaga ttagataaaa
     5101 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     5161 cccgtaaact cgcccagaag cttggtgtag agcagcctac actgtattgg catgtaaaaa
     5221 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     5281 tttgcccttt aaaaggggaa agctggcaag attttttacg caataacgct aaaagtttta
     5341 gatgtgcttt actaagtcat cgcaatggag caaaagtaca ttcagataca cggcctacag
     5401 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     5461 tagagaacgc gttatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     5521 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     5581 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     5641 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtggat
     5701 ctgttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
     5761 aggcagggat gttctcacct aagcttagaa cctttaccaa aggtgatgcg gagagatggg
     5821 taagcggtac ctacgaaaaa gatatcaaca agttgaagcg agagcttgaa gaattaggtg
     5881 tcgaagcgaa taccgcaagt tatagaaatc gtggagaaag atctgaactt gccgcttcta
     5941 ataatcaagt tacccatatt gatcaggaac atccctctca aaccaaatct acttctcagc
     6001 ctccaagtaa ccatctacct gcctttcagc aaatccctcc aattcaccaa tcagcttatc
     6061 ctcaaaataa tgttgctgaa gtattgatgc ctccaattcc tccttcagta gaggcttcta
     6121 gcgggcaaaa ttttaatcag ggaattgctt ctcaaaatcc cgcgatatct acatccaatt
     6181 tacctagtac tacaccttta tatatccctc ctgttaatta cggagcaaat caggtttctc
     6241 aacaaccaaa tcctcaattg cctggtgttt ccaattatta taatccaagt gctacttcaa
     6301 aacctgctgt gaatgtccag ccaccgcgta ttcctaccaa agcaactcct tcggctgaac
     6361 cttctatgac tgcttctgcg aatgctggtt ctatatccca agcgggtcct gatggagaat
     6421 atcagggaag agaacagatt gctccagttt cagatacaga agctgctaga aagacaactt
     6481 ctcagagttg gtacgtcact tataacccag catgcaaaag ggtttttaat attaatctcg
     6541 tgcacacact agagcatccc agtgttgttt gctgcgttaa gtttagtaac aatggcaagt
     6601 atttagcaac tggctgtaac caggccgcta atgtctttga tgttcagact ggcaaaaaac
     6661 tttttacttt gcatgaagaa tcacctgatc cttcccgtga tttgtatgtg agaaccattg
     6721 ctttcagccc tgatggtaag tatttagtca cgggaacaga agatcgtcaa ataaaactct
     6781 gggatctctc aactcaaaaa gttcgttacg tattttcagg tcatgaacaa gatatctatt
     6841 ccttagattt tagccataat gggcgattca tcgtctctgg tagtggcgat cgaactgcac
     6901 gactctggga tgtggagaca ggtcaatgta ttttaaagtt agaaattgag aatggtgtga
     6961 cggcaatagc catctctcca aatgatcaat tcattgcagt tggtagtcta gatcagataa
     7021 ttcgagtatg gtctgtgtcg ggtactttgg ttgaacgatt ggaaggtcat aaggagagtg
     7081 tttattctat tgccttttcg cccgacagca gcattcttct tagtggaagt cttgataaga
     7141 ctatcaaagt ctgggaatta caagcaactc gttcagttgg cctttcagct attaagcctg
     7201 aaggtatttg caaagccacc tatacaggac atactgattt tgttctttca gtagctgttt
     7261 cacccgacag ccgttgggga ttaagcggat cgaaggatag aagtatgcaa ttctgggatt
     7321 tgcaaacagg ccaatcttat ctcacttgtc aaggacacaa gaattcagtt atttccgtat
     7381 gctttagccc agatggtagg caatttgctt cgggaagtgg tgatttacgt gctcgcatat
     7441 ggtcaattga ccctgcatct ccttgaggta ccgagctcga attcactggc cgtcgtttta
     7501 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7561 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7621 cgcagcctga atggcgaatg gcgcccgcgc cacttctaaa taagcgaatt tcttatgatt
     7681 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     7741 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     7801 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
     7861 tagggggtgc aaataggttt taaaattgct ccaatcacat gttcttaaga aaaaaacgtc
     7921 aaaagaaatc taagtattcc caaaataatt cctcctgaca tgagaagaat aaaaaacact
     7981 aagatgcatt gtcagtatga aacacaagcc aaagttgcta acaataggat caattcttac
     8041 ttactggcaa tgttacgagg aatcttggta ggaggatgtt tggcaacata acgttctttt
     8101 gaatttgttt ttacacgacg agtaatgttc ttctgaaact gtgtgtttgc acgacgctga
     8161 gagggagttt gaaccgtcta aaagacatag ttaggatcca tgatgcacag atttctaaag
     8221 ataaaactac ttacaggcat gttggtttat taaaaaagaa aaacaggaat gataagaggg
     8281 aaagaatacg tgtacagagc aagtgtggaa agatatgctg aagacgtttg tatgaacaat
     8341 gccaagctgt aaagctagac tttcaaagta cacactaata cgttatacta tataccaaac
     8401 aaaagggtac gaacttacag tgtttaccaa tgtcagcaat actacactac gctatgatac
     8461 actacgttgt gtatcactat atgtcacatg ttctaattat atatcgtacc atgcccaacc
     8521 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8581 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8641 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9181 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10501 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10561 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10621 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10681 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10741 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
    10801 taaccttatg tatcatacac atacgattta ggtgacacta ta
//
