LOCUS       sequence-54342-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72110-BBa_J72097
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    162..186
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(210..253)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
       61 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      121 ttaaaaggat ctaggtgaag atccttgaat tgccattatc aacaagtttg tacaaaaaag
      181 caggctccga attggtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      241 taaggatgat ttctggaatt catgagatct catggcgaat taatctttct gcgaattgag
      301 atgacgccac tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt
      361 actatcttca aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag
      421 aagatattgc gcatgacaca ctctgacctg tcgcagatat tgattgatgg tcattccagt
      481 ctgctggcga aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt
      541 atccagcgca aagggacttt tcaggctagc cgccagccgg gtaatcagct tatccagcaa
      601 cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat
      661 caccaactgc ccgaacagca actcagccat ttcgttagca aacggcacat gctgactact
      721 ttcatgctca agctgaccga taacctgccg cgcctgcgcc atccccatgc tacctaagcg
      781 ccagtgtggt tgccctgcgc tggcgttaaa tcccggaatc gccccctgcc agtcaagatt
      841 cagtttcaga cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc
      901 gtaggagtgt ttatcgtcag catgaatgta aaagagatcg ccacgggtaa tgcgataagg
      961 gcgatcgttg a
//
