LOCUS       sequence-54350-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of blue-green emission ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..73
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     286..307
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(671..690)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
       61 tcggctgagc tcaccagaga acgtgtatat aaaggccgac gagcagaaga acggcatcaa
      121 ggcgtacttc aagatccgcc acaacatcga gggcggcggc gtgcagctcg cctaccacta
      181 ccagcagatc acccccatag gcgacggccc cgtgctgctg cccgacaacc actacctgag
      241 cgtgcagtcc atactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      301 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gtggcagcgg
      361 tggcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc aggtcgagct
      421 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac
      481 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      541 caccctcgtg accaccctgt cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      601 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat
      661 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      721 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      781 gcacaagctg gagtacagca cgcgtgacca actgactgaa gagcagatcg cagaatttaa
//
