LOCUS       sequence-54363-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Wu B, Chao JA, Singer RH
  TITLE     Fluorescence fluctuation spectroscopy enables quantitative imaging 
            of single mRNAs in living cells.
  JOURNAL   Biophys J. 2012 Jun 20;102(12):2936-44. Epub 2012 Jun 19.
  PUBMED    22735544
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2012-06-20"; volume: "102(12)"; pages: "2936-44. Epub 
            2012 Jun 19"
COMMENT     Sequence Label: phage-ubc-nls-ha-tdPCP-gfp
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(774..785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(786..812)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(786..812)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(816..836)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accatggtgg cgaccggtgg acggcccagc ggcacaaggt tgacgacaag atcttcgacc
       61 tgcgaggtcg cgacgaggga cttggtcaaa tcgtacaacg atttgcgcga ggcctcggtg
      121 ctattcgcaa cgattgtcac gtcgtgcgac catacctgag tgtagcgcac tttcggaagt
      181 ccggaatcaa cgacgtccgc ctgatccagt tttaggttga cgcgatacgc ggtcttggct
      241 ccgttttgac ggagcgaagc cgtgaggcgc agccgaccca ccagaggccc gaccttctct
      301 tcgaagatct gacggtctgc ggtggactgg atctcagtca gagtgcgagt agcctcgccg
      361 accgaaagaa cgatggtttt ggaggctagc ggatccgcac ggcccagcgg cacaaggttg
      421 acgacaagat cttcgacctg cgaggtcgcg acgagggact tggtcaaatc gtacaacgat
      481 ttgcgcgagg cctcggtgct attcgcaacg attgtcacgt cgtgcgacca tacctgagtg
      541 tagcgcactt tcggaagtcc ggaatcaacg acgtccgcct gatccagttt taggttgacg
      601 cgatacgcgg tcttggctcc gttttgacgg agcgaagccg tgaggcgcag ccgacccacc
      661 agaggcccga ccttctcttc gaagatctga cggtctgcgg tggactggat ctcagtcaga
      721 gtgcgagtag cctcgccgac cgaaagaacg atggttttgg aggctagcat atggcggcct
      781 tcgatggcgt agtcgggcac gtcgtagggg tagccaactt ttcttttctt ttttggg
//
