LOCUS       sequence-54367-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pDisplay-mSA-EGFP-TM
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..162)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(226..247)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     575..594
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     834..855
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aagcatgatg aggatgataa gggagatgat ggtgagcacc accagggcca ggatggctga
       61 gatcaccacc accttaaagg gcaaggagtg tggcaccacg atgacctcct gcgtgtcctg
      121 gcccacagca ttcagatcct cttctgagat gagtttttgt tcgtcgacct gcagccgcgg
      181 ccgcttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      241 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      301 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      361 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      421 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      481 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      541 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      601 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      661 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
      721 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      781 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      841 gtccagctcg accaggatgg gcaccacccc ggtgaaca
//
