LOCUS       sequence-54372-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pYD1-mSA
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             305..316
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             317..343
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             407..748
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             770..793
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctacttcat acattttcaa ttaagatgca gttacttcgc tgtttttcaa tattttctgt
       61 tattgcttca gttttagcac aggaactgac aactatatgc gagcaaatcc cctcaccaac
      121 tttagaatcg acgccgtact ctttgtcaac gactactatt ttggccaacg ggaaggcaat
      181 gcaaggagtt tttgaatatt acaaatcagt aacgtttgtc agtaattgcg gttctcaccc
      241 ctcaacaact agcaaaggca gccccataaa cacacagtat gtttttaagg acaatagctc
      301 gacgattgaa ggtagatacc catacgacgt tccagactac gctctgcagg ctagtggtgg
      361 tggtggttct ggtggtggtg gttctggtgg tggtggttct gctagcgcgg aagcgggtat
      421 caccggcacg tggtacaacc agtctggttc taccttcacc gttaccgcgg gtgcggacgg
      481 taacctgacc ggtcagtacg aaaaccgtgc gcagggcact ggttgccaga actctccgta
      541 caccctgacc ggtcgttaca acggtaccaa actggaatgg cgtgttgaat ggaacaactc
      601 taccgaaaac tgccactctc gtaccgaatg gcgtggtcag taccagggtg gtgcggaagc
      661 gcgtatcaac acccagtgga acctgaccta cgaaggtggt tctggtccgg cgaccgaaca
      721 gggtcaggac accttcacca aagttaaacc gtctgcggcg tctggatccg actacaagga
      781 cgatgacgac aagtaactcg agatctgata acaacagtgt agatgtaaca aaatcgactt
      841 tgttcccact gtacttttag ctcgtacaaa ataca
//
