LOCUS       sequence-54373-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pYD1-mSA
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(41..321)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(342..361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     461..483
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(521..539)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt
       61 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc
      121 cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc tccggatcaa
      181 gattgtacgt aaggtgacaa gctatttttc aataaagaat atcttccact actgccatct
      241 ggcgtcataa ctgcaaagta cacatatatt acgatgctgt tctattaaat gcttcctata
      301 ttatatatat agtaatgtcg tgatctatgg tgcactctca gtacaatctg ctctgatgcc
      361 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      421 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      481 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
      541 ttataggtta atgtcatgat aataatggtt tcttagacgg atcgcttgcc tgtaacttac
      601 acgcgcctcg tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt
      661 tttatgtttt gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg
      721 aagaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat
      781 atttattaga caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat
      841 gtaaaatcac aggattttcg tgtgtggtct tctac
//
