LOCUS       sequence-54376-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection, V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX304
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..224
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(265..570)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(367..386)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca ttactacgta
       61 gaatcgagac cgaggagagg gttagggata ggcttaccaa ccactttgta caagaaagct
      121 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      181 cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac ctgcagactg
      241 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
      301 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
      361 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
      421 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
      481 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
      541 tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag
      601 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
      661 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga
      721 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
      781 acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt
      841 cttataccgc aaaaatcagc gcg
//
