LOCUS       sequence-54377-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Kittleson JT, DeLoache W, Cheng HY, Anderson JC
  TITLE     Scalable plasmid transfer using engineered P1-based phagemids.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. 
            Epub 2012 Aug 30.
  PUBMED    23656280
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300054p";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "583-9"
COMMENT     Sequence Label: BBa_J72113-BBa_J72152
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     159..176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(209..236)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(252..323)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcttattaat cagataaaat atttctagag gcctcccctg attctgtgga taaccgggat
       61 ctgtaaggat caaccacttt gtacaagaaa gctgggtcga attgattacc gcctttgagt
      121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      181 cctgcacgtc gagttaggat cctataaacg cagaaaggcc cacccgaagg tgagccagtg
      241 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      301 gactgagcct ttcgttttat ttgatgcctg gagatcctca tttgtacagt tcatccatac
      361 catgcgtgat gcccgctgcg gttacgaact ccagcagaac catatgatcg cgtttctcgt
      421 tcggatcttt agacagaacg ctttgcgtgc tcagatagtg attgtctggc agcagaacag
      481 gaccatcacc gattggagtg ttttgctggt agtgatcagc cagctgcacg ctgccatcct
      541 ccacgttgtg gcgaatttta aaattcgctt taatgccatt tttttgttta tcggcggtga
      601 tgtaaacatt gtggctgtta aaattgtatt ccagcttatg gcccaggata ttgccgtctt
      661 ctttaaagtc aatgcctttc agctcaatgc ggtttaccag ggtatcgcct tcaaatttca
      721 cttccgcacg cgttttgtac gtgccgtcat ccttaaagga aatcgtgcgt tcctgcacat
      781 agccttccgg catggcggac ttgaagaagt catgctgctt catatggtcc ggataacgag
      841 caaagcactg aacaccataa gtcagcgtcg ttaccagagt cggccaaggt accggcagtt
      901 taccagtagt acagatgaac ttcagcgtca gtttaccatt agttgcgtca ccttcaccct
      961 cgccacgc
//
