LOCUS       sequence-54401-e         217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS
  TITLE     Regulatable vectors for environmental gene expression in 
            alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2012 Oct;78(19):7137-40. Epub 2012 Jul 20.
  PUBMED    22820336
REFERENCE   2  (bases 1 to 217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2012-10"; volume: "78(19)"; pages: 
            "7137-40. Epub 2012 Jul 20"
COMMENT     Sequence Label: pLMB509
FEATURES             Location/Qualifiers
     source          1..217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      69..155
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actatacaaa catatgcatc atcaccatca tcactaagag ctctacaaat aatgaattcc
       61 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      121 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
      181 cgaagcaacg gcccggaggg tggcgggcag gacgccc
//
