LOCUS       sequence-54405-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pTriExEKAR2G1
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acccaacaca atatattata gttaaataag aattattatc aaatcatttg tatattaatt
       61 aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac catggcacac
      121 catcaccacc accacgggag cggggagcag aaactaatat cagaggagga tcttggctca
      181 ggttcaggat cctccaccga cggcatggac gagctgtaca agggtgctag cggaggaatg
      241 gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg
      301 aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag
      361 ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc
      421 tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc
      481 gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc
      541 atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac
      601 tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg
      661 cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg
      721 ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgtt
//
