LOCUS       sequence-54406-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pTriExEKAR2G2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acccaacaca atatattata gttaaataag aattattatc aaatcatttg tatattaatt
       61 aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac catggcacac
      121 catcaccacc accacgggag cggggagcag aaactaatat cagaggagga tcttggctca
      181 ggttcaggat ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag
      241 gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg
      301 atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc
      361 gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt
      421 gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg
      481 gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag
      541 agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaaggg tgctagcgga
      601 ggaatggtga gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc
      661 aagctgaaga tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag
      721 ggcaagccct acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg
      781 cccttctcct acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag
      841 taccccgacg acatccccaa ctacttcaag cagtccttcc cc
//
