LOCUS       sequence-54408-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pTriExRhoA2G
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..116
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             164..193
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca
       61 aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag
      121 gagatatacc atggcacacc atcaccacca ccacgggagc ggggagcaga aactaatatc
      181 agaggaggat cttggctcag gttcaggatc cggcggccac caccgcgttg acttcaagac
      241 catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg accaccgcat
      301 cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga gcgccgtggc
      361 ccgcaactcc accgacggca tggacgagct gtacaagggt gctagcggag gaatggtgag
      421 caagggcgag gagaccacaa tgggcgtaat caagcccgac atgaagatca agctgaagat
      481 ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg gcaagcccta
      541 cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc ccttctccta
      601 cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt accccgacga
      661 catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc gcaccatgac
      721 cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg aggactcctt
      781 catctacgag atacacctca agggcgagaa cttccccccc aacgg
//
