LOCUS       sequence-54410-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiEKAR2G1
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctgttttga cctccataga agacaccgac tctagaggat ccggagatat accatggcac
       61 accatcacca ccaccacggg agcggggagc agaaactaat atcagaggag gatcttggct
      121 caggttcagg atcctccacc gacggcatgg acgagctgta caagggtgct agcggaggaa
      181 tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc
      241 tgaagatgga gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca
      301 agccctacga cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct
      361 tctcctacga cattctgacc accgcgttcg cctacggcaa cagggccttc accaagtacc
      421 ccgacgacat ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca
      481 ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg
      541 actccttcat ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga
      601 tgcagaagaa gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg
      661 tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg cggcggccac caccgcgttg
      721 acttcaagac catctacagg g
//
