LOCUS       sequence-54413-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiRhoA2G
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..105
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctccatagaa gacaccgact ctagaggatc cggagatata ccatggcaca ccatcaccac
       61 caccacggga gcggggagca gaaactaata tcagaggagg atcttggctc aggttcagga
      121 tccggcggcc accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag
      181 ctgcccgact atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac
      241 aacaaggtga ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg catggacgag
      301 ctgtacaagg gtgctagcgg aggaatggtg agcaagggcg aggagaccac aatgggcgta
      361 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
      421 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
      481 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
      541 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
      601 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
      661 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
      721 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
      781 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
      841 gagggctccg gaatcctgga ggac
//
