LOCUS       sequence-54416-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Narain Y, Wyttenbach A, Rankin J, Furlong RA, Rubinsztein DC
  TITLE     A molecular investigation of true dominance in Huntington's disease.
  JOURNAL   J Med Genet. 1999 Oct;36(10):739-46.
  PUBMED    10528852
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Med 
            Genet."; date: "1999-10"; volume: "36(10)"; pages: "739-46"
COMMENT     Sequence Label: pHM6-Q74
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             437..454
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(467..485)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(468..485)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(505..522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    511..735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggag accatgtacc catacgacgt cccagactac gctggaagct ttatgaaggc
      121 cttcgagtcc ctcaagtcct tccagcagca gcagcagcag cagcagcagc agcagcagca
      181 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      241 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      301 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
      361 acagccgcca ccgccgccgc cgccgccgcc gcctcctcag cttcctcagc cgccgaattc
      421 gcggccgcta cacgtgcatc atcatcatca tcattaaggg ccctattcta tagtgtcacc
      481 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      721 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      781 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      841 ctacacttgc cagcgcccta gcgcc
//
