LOCUS       sequence-54418-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Conformation-Selective ATP-Competitive Inhibitors Control Regulatory
            Interactions and Noncatalytic Functions of Mitogen-Activated Protein
            Kinases.
  JOURNAL   Chem Biol. 2014 May 22;21(5):628-35. doi: 
            10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.
  PUBMED    24704509
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.02.016"; journalName: "Chem Biol"; date: 
            "2014-05-22- 22"; volume: "21"; issue: "5"; pages: "628-35"
COMMENT     Sequence Label: pcDNA3-eGFP-LIC
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..767)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             complement(57..767)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(99..120)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     448..467
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     707..728
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatagaata gttatccact tccaatattg gattggaagt acgatccctg aagctgcttg
       61 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      121 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      181 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      241 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      301 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      361 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      421 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      481 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      541 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      601 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      661 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      721 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctca
//
