LOCUS       sequence-54427-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     various new plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pin1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      728..775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccaa tgcggacgag gagaagctgc cgcccggctg
      121 ggagaagcgc atgagccgca gctcaggccg agtgtactac ttcaaccaca tcactaacgc
      181 cagccagtgg gagcggccca gcggcaacag cagcagtggt ggcaaaaacg ggcaggggga
      241 gcctgccagg gtccgctgct cgcacctgct ggtgaagcac agccagtcac ggcggccctc
      301 gtcctggcgg caggagaaga tcacccggac caaggaggag gccctggagc tgatcaacgg
      361 ctacatccag aagatcaagt cgggagagga ggactttgag tctctggcct cacagttcag
      421 cgactgcagc tcagccaagg ccaggggaga cctgggtgcc ttcagcagag gtcagatgca
      481 gaagccattt gaagacgcct cgtttgcgct gcggacgggg gagatgagcg ggcccgtgtt
      541 cacggattcc ggcatccaca tcatcctccg cactgagtga cattggaagt ggataacgga
      601 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      661 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      841 gcgggtgtgg tggttacgcg cagcgtgacc gcta
//
