LOCUS       sequence-54432-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of GFP (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCMV-GFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(128..331)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(157..177)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        332..707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc
       61 agaattcgaa gcttgagctc gagatctgag tccggtagcg ctagcggatc tgacggttca
      121 ctaaaccagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
      181 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
      241 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
      301 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
      361 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
      421 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
      481 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
      541 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      601 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
      661 ctaatgaccc cgtaattgat tactattact agtcaataat caatgtcgac c
//
