LOCUS       sequence-54436-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Gallegos LL, Kunkel MT, Newton AC
  TITLE     Targeting protein kinase C activity reporter to discrete 
            intracellular regions reveals spatiotemporal differences in 
            agonist-dependent signaling.
  JOURNAL   J Biol Chem. 2006 Oct 13. 281(41):30947-56.
  PUBMED    16901905
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2006 Oct 13. 281(41):30947-56."
COMMENT     Sequence Label: GolgiCFP
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..55)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(38..55)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(75..92)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    81..305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(438..457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcaggagag ctgtacagta atctagaggg ccctattcta tagtgtcacc taaatgctag
       61 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
      121 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
      181 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
      241 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
      301 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
      361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgt
//
