LOCUS       sequence-54486-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Joung
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JDS82
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..145
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      121 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca
      181 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      241 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      301 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      361 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      421 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      481 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      541 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt
      601 gaacagagac gattaatgcg tctcgctgac acccgaacag gtggtcgcca ttgcttccca
      661 cgacggagga cggccagcct tggagtccat cgtagcccaa ttgtccaggc ccgatcccgc
      721 gttggctgcg ttaacgaatg accatctggt ggcgttggca tgtcttggtg gacgacccgc
      781 gctcgatgca gtcaaaaagg gtctgcctca tgctcccgca ttgatcaaaa gaaccaaccg
      841 gcggattccc gagagaactt cccatcgagt cgcgggatcc caactagtca aaagtgaact
      901 ggagga
//
