LOCUS       sequence-54499-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Galvez-Santisteban M, Rodriguez-Fraticelli AE, Bryant DM, 
            Vergarajauregui S, Yasuda T, Banon-Rodriguez I, Bernascone I, Datta 
            A, Spivak N, Young K, Slim CL, Brakeman PR, Fukuda M, Mostov KE, 
            Martin-Belmonte F
  TITLE     Synaptotagmin-like proteins control the formation of a single apical
            membrane domain in epithelial cells.
  JOURNAL   Nat Cell Biol. 2012 Jul 22. doi: 10.1038/ncb2541.
  PUBMED    22820376
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2012 Jul 22. doi: 10.1038/ncb2541."
COMMENT     Sequence Label: pRVH1-hygro shStx3
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(105..121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(138..554)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(154..170)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(186..208)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ggattgggat cttataagtt ctgtatgaga ccacagatct ccgagtatgg agaggcctct
       61 tcaagagaga tgtgcctctc catactcttt ttggaaaagc ctatcgatac cgtcgacctc
      121 gagggatctt caattccggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga
      181 ggcggggtcg aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc
      241 ggaggcttag ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc
      301 caggtcacga tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg
      361 atcttaacct gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta
      421 aaggttggcc attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac
      481 tggttgtgag cgatccgctc gacatctttc cagtgaccta aggtcaaact taanggagtg
      541 gtaacagtct ggcccta
//
