LOCUS       sequence-54507-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Furlong RA, Narain Y, Rankin J, Wyttenbach A, Rubinsztein DC
  TITLE     Alpha-synuclein overexpression promotes aggregation of mutant 
            huntingtin.
  JOURNAL   Biochem J. 2000 Mar 15;346 Pt 3:577-81.
  PUBMED    10698681
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "2000-03-15"; volume: "346 Pt 3"; pages: "577-81"
COMMENT     Sequence Label: pHM6-alphasynuclein-A53T
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             557..574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(587..605)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(588..605)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(625..642)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    631..742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggag accatgtacc catacgacgt cccagactac gctggaagct tgatggatgt
      121 attcatgaaa ggactttcaa aggccaagga gggagttgtg gctgctgctg agaaaaccaa
      181 acagggtgtg gcagaagcag caggaaagac aaaagagggt gttctctatg taggctccaa
      241 aaccaaggag ggagtggtgc atggtgtgac aacagtggct gagaagacca aagagcaagt
      301 gacaaatgtt ggaggagcag tggtgacggg tgtgacagca gtagcccaga agacagtgga
      361 gggagcaggg agcattgcag cagccactgg ctttgtcaaa aaggaccagt tgggcaagaa
      421 tgaagaagga gccccacagg aaggaattct ggaagatatg cctgtggatc ctgacaatga
      481 ggcttatgaa atgccttctg aggaagggta tcaagactac gaacctgaag ccgagaattc
      541 gcggccgcta cacgtgcatc atcatcatca tcattaaggg ccctattcta tagtgtcacc
      601 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      661 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      721 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctgggggg
//
