LOCUS       sequence-54528-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-VSFP-CR
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      769..778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgctcccat tcgacgcttg ttctgtgata ttgttcttct gcttgaagcc ttcatttgtt
       61 ggtgggaata aaatattctt gctaatcgaa ccacacgcag caaacgggcc aacacaacca
      121 actgccccaa accatcggct cctgtttctt gcacgtattc atctaacaca gtgtaaaata
      181 tcgtgacgac gaatgtgaca acgataatca aaccatcagc aacctcccag gggttggtga
      241 agaaattctt gggcccgtag gcaaatatcc ttaatcctaa atccagcatg aaataacaag
      301 aaagagccaa agccatcccg tcataaaagg attgtgaaga ttcacttttt cctggaagac
      361 tgagatcaat gatcatgagg atgatgtcca agaaaattag gaagactcca aagactcgca
      421 tccctagatg atcaatcact gctcggacac gaaactggac gcgacctaca ccagtagtag
      481 gtatctccat tctcccatcg tccactccat gttcattctc gccccaccat agacctgaaa
      541 tctcgggtat atctattcgt tcctcctgtc ttgtctctgg tttttcactc ggtccttggt
      601 ctacatcatt ccaatgaact ttctttaccg attcactttt tcttggcgca gctttcggcg
      661 gagcagtgac gtcaccattt atcgtgacgt caacgacgtt ccgcatgact gcaccgtcaa
      721 cgccaactaa atcagctgga ggactaaaat ctgaaccgtc gaatccctcc atggtggcgc
      781 tagctcatgc agatctcagt ggtatttgtg agccagggca ttggccacac ca
//
