LOCUS       sequence-54559-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Dantas G, Kuhlman B, Callender D, Wong M, Baker D
  TITLE     A large scale test of computational protein design: folding and 
            stability of nine completely redesigned globular proteins.
  JOURNAL   J Mol Biol. 2003 Sep 12. 332(2):449-60.
  PUBMED    12948494
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Sep 12. 332(2):449-60."
COMMENT     Sequence Label: URN
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..96
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             307..324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(377..395)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      391..438
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      475..855
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(562..581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     772..793
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gaaagaaacc
       61 gctgctgcta aattcgaacg ccagcacatg gacagcccag atctgggtac ctccggtaca
      121 actttgttcg ttgcaacctc tccttacgag tccaccactg ataatgactt accgttccgc
      181 aaaggtgaca aaatctggat tgaagataac gcaccgggtg attactggaa agccgtttca
      241 tcaacgacag gcaaaaccgg gtacattcct gcagacaaaa ttcgcccagc aggcgctggt
      301 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      361 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      421 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      481 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      541 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      601 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      661 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      721 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      781 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggaa
      841 ttttgccgat ttcggcctat tggttaaaaa atgagctgat tacaaattac gcgatttaca
      901 at
//
