LOCUS       sequence-54573-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T
  TITLE     Translation elongation can control translation initiation on 
            eukaryotic mRNAs.
  JOURNAL   EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 
            2013 Dec 19.
  PUBMED    24357599
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201385651"; journalName: "EMBO J"; date: "2014-01-1- 
            1"; volume: "33"; issue: "1"; pages: "21-34"
COMMENT     Sequence Label: 721-2µ-staHIS3
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(185..211)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(212..868)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="TEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAEQ
                     ATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAFK
                     EALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 ataataaaaa tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta
       61 ccaacgattt gacccttttc catcttttcg taaatttctg gcaaggtaga caagccgaca
      121 accttgattg gagacttgac caaacctctg gcgaagaagt ccaaagcttg catgcctgca
      181 gctacgcgta gtccgggacg tcgtacgggt acataagaac acctttggtg gagggaacat
      241 cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac
      301 tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg gtaattctgc
      361 tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga tctcctactt
      421 tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga tctaccaccg
      481 ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt actccacgca
      541 cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct tcagtggtgt
      601 gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag ccggaatgct
      661 tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg ttaatcactt
      721 gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag atcgagtgct
      781 ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt tcatttgtaa
      841 tacgctttac tagggctttc tgctctgtca
//
