LOCUS       sequence-54588-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of enzyme-dead UTX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K
  TITLE     UTX regulates mesoderm differentiation of embryonic stem cells 
            independent of H3K27 demethylase activity.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4.
  PUBMED    22949634
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Sep 4."
COMMENT     Sequence Label: pCS2-UTX-F-MT2
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             515..538
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    571..705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(595..614)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     649..668
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tattggctgg tgcaacaaca ttgcttggaa tgttggtcca cttacagcct gccagtataa
       61 attggcagtg gaacggtacg aatggaacaa attgcaaagt gtgaagtcaa tagtacccat
      121 ggttcatctt tcctggaata tggcacgaaa tatcaaggtc tcagatccaa agctttttga
      181 aatgattaag tattgtcttc taagaactct gaagcaatgt cagacattga gggaagctct
      241 cattgctgca ggaaaagaga ttatatggca tgggcggaca aaagaagaac cagctcatta
      301 ctgtagcatt tgtgaagtgg aggtttttga tctgcttttt gtcactaatg agagtaattc
      361 acgaaagacc tacatagtac attgccaaga ttgtgcacga aaaacaagcg gaaacttgga
      421 aaactttgtg gtgctagaac agtacaaaat ggaggacctg atgcaagtct atgaccaatt
      481 tacattagct cctccattac catccgcctc atctgactac aaggacgacg atgacaagtg
      541 atctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
      601 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      661 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      721 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
      781 gcgccaatgc attgggcccg gtacccagct ttgtccctta gtgaggtaat gc
//
