LOCUS       sequence-54596-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Geraldo S, Khanzada UK, Parsons M, Chilton JK, Gordon-Weeks PR
  TITLE     Targeting of the F-actin-binding protein drebrin by the microtubule 
            plus-tip protein EB3 is required for neuritogenesis.
  JOURNAL   Nat Cell Biol. 2008 Oct;10(10):1181-9. Epub 2008 Sep 21.
  PUBMED    18806788
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2008-10"; volume: "10(10)"; pages: "1181-9. Epub 2008
            Sep 21"
COMMENT     Sequence Label: His-drebrin
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(329..346)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             365..387
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    402..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(426..445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(427..445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(533..552)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttagcagtg gctggcacaa gcgcacttgc gggcatcagg cacatcttcg cccacccagt
       61 tgatgagcac gtattttggc agagcagctt gggagtcctt gacactgcag aagccgtaca
      121 tcaccttctg gttctcaaag tgtcccgaaa gctcctgcaa gcccccttct cctgatgctg
      181 caagcttgag gtcatcggag ccatcttcat atgtgtacag agcccagtcg gccgcgctct
      241 cctctcggat cacctcctcg taagccgcca gcagctccag gcggtggccg ctgaagctga
      301 cgccggccat cgaattcgga tcctggctgt ggtgatgatg gtgatggctg ctgcccatgg
      361 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      421 aattccccta tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac
      481 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      541 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      601 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      661 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      721 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac
      781 ctttcgcggt atggcatgat agcgccc
//
