LOCUS       sequence-54611-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-terminal tal protein segments 
            increases mutation induction; Fok I DD (improved 
            version=pCS2TAL3-DDD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-DD
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..23)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3..21)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(42..58)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(42..58)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(55..77)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    135..156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
       61 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      181 ttccaccggt cgtctccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
      241 ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc
      301 gccgtattgg cgaacgcacg tcccatcgcg ttgccggatc ccaactagtc aaaagtgaac
      361 tggaggagaa gaaatctgaa cttcgtcata aattgaaata tgtgcctcat gaatatattg
      421 aattaattga aattgccaga aattccactc aggatagaat tcttgaaatg aaggtaatgg
      481 aattttttat gaaagtttat ggatatagag gtaaacattt gggtggatca aggaaaccgg
      541 acggagcaat ttatactgtc ggatctccta ttgattacgg tgtgatcgtg gatactaaag
      601 cttatagcgg aggttataat ctgccaattg gccaagcaga tgaaatgcaa gactatgtcg
      661 aagaaaatca aacacgaaac aaacatatca accctaatga atggtgg
//
