LOCUS       sequence-54614-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-term tal protein segments 
            increases mutation induction; Fok I RR (improved version = 
            pCS2TAL3-RRR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-RR
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     40..57
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             123..212
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     782..804
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     796..813
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     797..813
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     823..842
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        823..841
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac actatagaat
       61 acaagctact tgttcttttt gcaggatctg ccaccatggc tccaaagaag aagcgtaagg
      121 tatacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag
      181 gatcgtatcc atatgacgtt ccagattacg ctgctcatgg taccgtggat ctacgcacgc
      241 tcggctacag tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc
      301 agcaccacga ggcactggtg ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc
      361 aacacccggc agcgttaggg accgtcgctg tcacgtatca gcacataatc acggcgttgc
      421 cagaggcgac acacgaagac atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc
      481 tggaggcctt gctcacggat gcgggggagt tgagaggtcc gccgttacag ttggacacag
      541 gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcat
      601 cgcgcaatgc actgacgggt gcccccctgg agacgggcgc cgctacaggg cgcgtcccat
      661 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      721 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
      781 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
      841 ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc tttagtg
//
