LOCUS       sequence-54617-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-term tal protein segments 
            increases mutation induction; Fok I RR (improved version = 
            pCS2TAL3-RRR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-RR
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..24)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4..22)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(43..59)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(43..59)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(56..78)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    67..83
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    136..157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
       61 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      181 tttccaccgg tcgtctccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct
      241 gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat
      301 cgccgtattg gcgaacgcac gtcccatcgc gttgccggat cccaactagt caaaagtgaa
      361 ctggaggaga agaaatctga acttcgtcat aaattgaaat atgtgcctca tgaatatatt
      421 gaattaattg aaattgccag aaattccact caggatagaa ttcttgaaat gaaggtaatg
      481 gaatttttta tgaaagttta tggatataga ggtaaacatt tgggtggatc aaggaaaccg
      541 gacggagcaa tttatactgt cggatctcct attgattacg gtgtgatcgt ggatactaaa
      601 gcttatagcg gaggttataa tctgccaatt ggccaagcac gtgaaatgca acgatatgtc
      661 gaagaaaatc aaacacgaaa caaacatatc aaccctaatg aatggtggaa agtctatcca
      721 tcttctgtaa cggaatttaa gtttttattt gtgagtggtc actt
//
