LOCUS       sequence-54621-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_wt_Venus_wt
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..119
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
       61 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
      121 aaggagatat accatggcac accatcacca ccaccacggg agcggggagc agaaactaat
      181 atcagaggag gatcttggct caggttcagg atccgtgagc aagggcgagg agaccacaat
      241 gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca
      301 cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa
      361 cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt
      421 cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca
      481 gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca agggcatcgt
      541 gaaggtgaag tccgacatct ccatggagga ggactccttc atctacgaga tacacctcaa
      601 gggcgagaac ttccccccc
//
