LOCUS       sequence-54632-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_105_Venus_229
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        58..167
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             188..205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             215..244
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt ccccggagtt aatccgggac
       61 ctttaattca acccaacaca atatattata gttaaataag aattattatc aaatcatttg
      121 tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac
      181 catggcacac catcaccacc accacgggag cggggagcag aaactaatat cagaggagga
      241 tcttggctca ggttcaggat ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc
      301 catggaggag gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa
      361 cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt
      421 gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca
      481 ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta
      541 tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac
      601 cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaaggg
      661 tgctagcgga ggaatggtga gcaagggcga ggagaccaca atgggcgtaa tcaagcccga
      721 catgaagatc aagctgaaga tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg
      781 cgagggcgag ggcaagccct acgacggcac caacaccatc aacctggagg tgaaggaggg
      841 agcccccctg cccttctcct acgacattct gaccaccgcg ttcgcctacg gcaacaggg
//
