LOCUS       sequence-54641-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_159_Venus_173
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..120
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             168..197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttaatccggg acctttaatt caacccaaca caatatatta tagcttaaat aagaattatt
       61 atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt atttacaatc
      121 aaaggagata taccatggca caccatcacc accaccacgg gagcggggag cagaaactaa
      181 tatcagagga ggatcttggc tcaggttcag gatccgacgg cgtgctgaag ggcgacgtca
      241 agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag accatctaca
      301 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc
      361 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact
      421 ccaccgacgg catggacgag ctgtacaagg gtgctagcgg aggaatggtg agcaagggcg
      481 aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca
      541 acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca
      601 ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc
      661 tgaccaccgc gttcgcctac ggcaacaggg
//
