LOCUS       sequence-54673-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design1_mTFP_227_Venus_229
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..119
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             167..196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
       61 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
      121 aaggagatat accatggcac accatcacca ccaccacggg agcggggagc agaaactaat
      181 atcagaggag gatcttggct caggttcagg atcctccacc gacggcatgg acgagctgta
      241 caagggtgct agcggaggaa tggtgagcaa gggcgaggag accacaatgg gcgtaatcaa
      301 gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg ccttcgtgat
      361 cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc tggaggtgaa
      421 ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg cctacggcaa
      481 cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt ccttccccga
      541 gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc
      601 cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg gcgagaactt
      661 cccccccaa
//
