LOCUS       sequence-54674-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_wt_Venus_wt
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..143
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             164..181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             191..220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tgagcggata acaattcccc ggagttaatc cgggaccttt aattcaaccc aacacaatat
       61 attatagtta aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg
      121 taaattacat tttatttaca atcaaaggag atataccatg gcacaccatc accaccatca
      181 cgggagcggg gagcagaaac taatatcaga ggaggatctt ggctcaggaa ccggtagatc
      241 tgggcacatg gcggacgagg agaagctgcc gcccggctgg gagaagcgca tgagccgcag
      301 ctcaggccga gtgtactact tcaaccacat cactaacgcc agccagtggg agcggcccag
      361 cggcaacagc agcagtggtg gcaaaaacgg gcagggggag cctgccagga agctgcgcat
      421 tctgcagtcg actagggagc aagggcttgt gggatccgtg agcaagggcg aggagaccac
      481 aatgggcgta atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg
      541 ccacgccttc gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat
      601 caacctggag gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc
      661 gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa
      721 gcagtccttc cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat
      781 cgtgaaggtg aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct
      841 caagggcgag aacttccccc ccaacggcc
//
